Changes in translational efficiency is a dominant regulatory mechanism in the environmental response of bacteria Journal Article


Authors: Taylor, Ronald C.; Robertson, Bobbie-Jo M. Webb; Markillie, Lye Meng; Serres, Margrethe H.; Linggi, Bryan E.; Aldrich, Joshua T.; Hill, Eric A.; Romine, Margaret F.; Lipton, Mary S.; Wiley, H. Steven
Article Title: Changes in translational efficiency is a dominant regulatory mechanism in the environmental response of bacteria
Abstract: To understand how cell physiological state affects mRNA translation, we used Shewanella oneidensis MR-1 grown under steady state conditions at either 20% or 8.5% O-2. Using a combination of quantitative proteomics and RNA-Seq, we generated high-confidence data on gt; 1000 mRNA and protein pairs. By using a steady state model, we found that differences in protein-mRNA ratios were primarily due to differences in the translational efficiency of specific genes. When oxygen levels were lowered, 28% of the proteins showed at least a 2-fold change in expression. Transcription levels were sp. significantly altered for 26% of the protein changes; translational efficiency was significantly altered for 46% and a combination of both was responsible for the remaining 28%. Changes in translational efficiency were significantly correlated with the codon usage pattern of the genes and measurable tRNA pools changed in response to altered O-2 levels. Our results suggest that changes in the translational efficiency of proteins, in part due to altered tRNA pools, is a major determinant of regulated alterations in protein expression levels in bacteria.
Keywords: RIBOSOMAL-RNA; ESCHERICHIA-COLI; MESSENGER-RNA; GENE-EXPRESSION; ACID; ACCURATE MASS; CODON USAGE; SHEWANELLA-ONEIDENSIS MR-1; PROTEIN EXPRESSION; URIDINE-5-OXYACETIC; PROTEOMIC ANALYSES
Journal Title: Integrative Biology
Volume: 5
Issue: 11
ISSN: 1757-9694
Publisher: Royal Society of Chemistry  
Publication Place: CAMBRIDGE; THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
Date Published: 2013
Start Page: 1393
End Page: 1406
DOI/URL:
Notes: PT: J; TC: 0; UT: WOS:000326052600009