Localization of rRNA transcribed spacer domains in the nucleolinus and maternal procentrosomes of surf clam (Spisula) oocytes Journal Article


Authors: Alliegro, Mark C.; Alliegro, Mary Anne
Article Title: Localization of rRNA transcribed spacer domains in the nucleolinus and maternal procentrosomes of surf clam (Spisula) oocytes
Abstract: The nucleolinus is a nuclear subcompartment long ago posited to play a role in cell division. In a recent study using surf clam oocytes, cytoplasmic foci containing a nucleolinar protein were shown to later recruit gamma-tubulin, identifying them as centrosomal precursors. 1 We now demonstrate the presence of structural RNAs from the nucleolinus in these procentrosomes. They include the well-known but poorly understood rRNA-transcribed spacer regions. In situ hybridization revealed a specific and dynamic association of these structural RNAs with the cell division apparatus that extends through the early stages of meiosis. In addition to their bearing on the debate over the nature of centrosome- and spindle-associated RNAs, the observations also suggest that rRNA spacer regions are not simply waste products to be discarded immediately, but may be functional byproducts that play a role in formation of the cell division apparatus.
Keywords: DNA; internal transcribed spacer; SPINDLE; microtubule organizing center; MTOC; centrosomal RNA; BODIES; PLASMODIUM; p53; ITS; monoclonal antibody; ETS; external transcribed spacer; SRP; recognition particle; mAb; MDM2
Journal Title: RNA Biology
Volume: 10
Issue: 3
ISSN: 1547-6286
Publisher: Landes Bioscience  
Publication Place: AUSTIN; 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA
Date Published: 2013
Start Page: 367
End Page: 372
DOI/URL:
Notes: PT: J; TC: 0; UT: WOS:000316784400006
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